Abstract

IN VITRO EFFICACY OF NANOPARTICLES AGAINST MYCOBACTERIUM TUBERCULOSIS

Shahnawaz Mohd., Mohammad Shameem* and Shamsul Hayat

ABSTRACT

Tuberculosis (TB) is an endemic disease in all over the world, affecting both animals and humans. The present work intends to evaluate the antimycobacterial activity of Silver nanoparticles (AgNPs), Zinc Oxide nanoparticles (ZnONPs) and Gold nanoparticles (AuNPs) by measuring the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) using the Micro plate Alamar Blue Assay (MABA). The reference strain of Mycobacterium tuberculosis H37Rv was tested. The results revealed that the AgNPs showed sensitivity against M. tuberculosis strain at a concentration of 1.6 μg/ml, while ZnONPs and AuNPs were sensitive at 12.6 μg/ml and 3.12 μg/ml respectively. However, the concentrations of 0.8 μg/ml, 1.6 μg/ml, 3.12 μg/ml, 6.25 μg/ml, 12.5 μg/ml and 25 μg/ml, of Ag and ZnO NPs were unable to kill the M. tuberculosis strains. Therefore, it can be inferred from the present observation that none of these doses demonstrated bactericidal effects on M. tuberculosis strains.

Keywords: Mycobacterium tuberculosis, Silver nanoparticle, Zinc Oxide nanoparticles, Gold nanoparticles, MABA, H37Rv.