Abstract

EVALUATION OF EFFECT OF WITHANIA COAGULANS EXTRACTS IN ANIMAL MODEL OF DIABETES MELLITUS

Anuj Kumar*, Sanjay Kumar, Akhilesh Kumar Rana and Amit Singh

ABSTRACT

Objective: To study the bioactivity of methanolic extract of Withania coagulanson in animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters. Methods: Diabetes mellitus was induced in rats by injecting streptozotocin (60 mg/kg) intraperitonealy. Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus. Rats having fasting blood glucose>250mg/dL were further selected for study and they were divided into four groups control, control+streptozotocin, streptozotocin+metformin (75mg/kg) and streptozotocin+extract of Withaniacoagulans). Each group consisted of six rats of eithersex. Metforminand experimental extract were administered for 21 day. Triglyceride, cholesterol level were measured on day 21 by commercially available kit. Blood glucose was measured on days 7 and 21. Anti-oxidant potential was assessed by estimating extent of lipidperoxidation (LPO) by malondialdehyde (MDA), nitricoxide (NO), superoxide dismutase (SOD) and glutathione (GSH) in liver, kidney, pancreas, muscle tissues on day 21. Unpaired and paired students t-test was applied for statistical analysis. Results: The extract of Withania coagulans showed significant decrease in blood glucose level on day 21. The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21. The treatment did not show statistically significant difference of cholesterol, but showed significant difference of triglycerides level on day 21. Conclusion: The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of Withania coagulans.

Keywords: .