Abstract

COMPARATIVE ANTI - INFLAMMATORY POTENTIAL OF BETALAINS OF HAIRY ROOT CULTURE OF BETA VULGARIS AND BETA VULGARIS

Ashoka Babu VL*, Shanaz Banu and Mohammad Azamthulla

ABSTRACT

The main objective of the present study was the production of betalains from hairy root culture of Beta vulgaris and comparative evaluation of its anti inflammatory potential with Beta vulgaris. Hairy roots were initiated using different strains of Agrobacterium Rhizogens such as A.4, A.2/83, A.20/83 and LMG 150; LMG 150 was found to initiate large number of hairy roots. Hairy roots were further grown on MS media and betalains was extracted with acidified water and its content was estimated spectrophotometrically. In -vitro anti-inflammatory activity of betalains from beetroot and hairy roots was evaluated by membrane stabilization and inhibition of denaturation of albumin methods. Betalains of hairy roots possess significant anti-inflammatory activity in a dose dependent manner, where as exhibited extremely significant anti-inflammatory activity (1000 μg/ml) when compared with betalains of Beta vulgaris.

Keywords: Beta vulgaris, Betalains, Agrobacterium rhizogens, Membrane stabilization.