Abstract

FORMULATION AND EVALUATION OF EZETIMIBE LOADED SOLID LIPID NANOPARTICLES

Chandraprakash Chilukuri* and S. Muhammad Fazal Ul Haq

ABSTRACT

Ezetimibe is an anti Hyperlipidaemic drug with low bioavailability (35%) and poor aqueous solubility (0-00846 mg/L.) The present study was focused to enhance the bioavailability of ezetimibe. Drug loaded solid lipid nanoparticles were prepared by using high shear homogenization and ultrasonication method using glycerol mono sterate as lipid and tween 80, poloxamer 188 as surfactants. Prepared solid lipid nanoparticles were characterized for particle size, surface morphology and thermal properties. Evaluation studies like entrapment efficiency, Invitro drug release studies, and stability studies. The optimized formulation of ezetimbe loaded solid lipid nanoparticles exhibited particle size (432 nm), zeta potential (-13.8mV) and poly dispersity index (0.168). Thermal property studies performed by using differential scanning calorimetry showed no chemical interaction between drug and lipid. Entrapment efficiency of optimized formulation was found to be 88.72+ 0.21 %.scanning electron microscope study revealed solid lipid nanoparticles were spherical in shape with smooth surface. invitro drug release studies showed a drug release of 89.2 + 0.2% for optimized formulation. Stability studies performed on the optimized formulation at different temperatures for 2 months revealed there is slight decrease in the stability of drug loaded solid lipid nanoparticles.

Keywords: solid lipid nanoparticles, high shear homogenization, ultrasonication, differential scanning calorimetry & scanning electron microscope.