Abstract

HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY FOR DETERMINATION OF DIFFERENT CLASSES OF DRUGS

Archana Gahtori, Taniya Mamgain* and Sweta Kothari

ABSTRACT

HILIC is a type of liquid chromatographic technology which is used for the separation of the polar compounds such as peptides, nucleic acid etc. HILIC combines the characteristics of three major liquid chromatographic method i.e. Reversed-phase Liquid chromatography, normal-phase Liquid chromatography, and ion-Exchange chromatography. This review paper represents the recent application of the HILIC method for the analysis and separation of different classes of pharmaceutical drugs including anti-diabetic drugs, antibiotics and cytostatic drugs. It shows the analysis of drugs in different matrix samples with different stationary and mobile phase and with the use of different detection method. HILIC overcomes the drawback of the unseparated small polar compounds, poor solubility of the non- polar samples with the mobile phase as in other chromatographic techniques.

Keywords: HILIC, retention mechanism, mobile phase, stationary phase, drug analysis.