Abstract

HPLC PROFILES OF METHANOL EXTRACTS OF SOME AMELIORATED MEDICINES BASED MEDICINAL PLANT EXTRACTS IN UTILISATION IN DEMOCRATIC REPUBLIC OF CONGO

Mbenza P. A., Nsangu M. J., Kimbeni M. T., Mbinze K. J., Mbala M., Mpuza K., Lami N. J. and Cimanga K. R.*

ABSTRACT

HPLC fingerprintings or chromatograms of four ameliorated medicines based medicinal plant extracts including Manadiar tablets and suspension based Mangifera indica and Psidium guayava stem bark used as antidiarrheal, Manalaria suspension and tablets based Nauclea latifolia and Cassia occidentalis leaves used to treat malaria were studied. The fingerprintings of Manadiar tablets and suspension showed the identification of two major compounds as mangiferine and quercetin at retention time of 30.567 and 59.033 minutes respectively. In Manadiar tablets the adopted or accepted high quantity of mangiferine was estimated to be 1.598 mg/tablet at the wavelength of 254 nm and that of quercetin was 0.880 mg/tablet at 280 nm while in Manadiar suspension, the high quantity of mangiferine adopted was 1.015 mg at 220 nm and that of quercetin was 0.134 mg/5 ml suspension at the same wavelength. These quantities measured at 220 and 254 nm in Manadiar tablets for mangiferine and quercetin respectively, were found in high quantities compared to other obtained at wavelengths of 220, 280 and 320 nm respectively as 1.540, 1.584 and 1.592 mg/tablet for mangiferine and 0.874, 0865 and 0.848 mg/tablet for quercetin while it was of 0.993, 0996 and 1.O12 for mangiferine and 0.110, 0.122 and 0.093 for quercetin in 5 ml suspension at these three wavelengths. In Manalaria tablets and suspension, fingerprintings showed also the detection of two major compounds vitexin and chrysophanol in both medicines at retention time of 2.767 and 62.513 minutes respectively, at different quantities. Indeed, vitexin and chrysophanol were found to be in high amounts of 2.111 and 1.289 mg respectively in Manalaria tablets/tablet and 5.720 and 0.852 mg/5 ml in Manalaria suspension recorded at 220 nm compared to other low amounts recorded at 254, 280 and 320 nm of 1.696, 4.320 and 2.520 for vitexin and 0.038, 0.026 and 00.15 mg/5 ml Manalaria suspension for chrysophanol while they were at 1.744, 1.276 and 0.666 mg/tablet for vitexin and 0.920, 0.780 and 0783 mg/tablet for chrysophanol in Manalaria tablets. The fingerpritings of these four ameliorated medicines were uniforme and homogene althouth some minor compounds were detected without no significant effects on the detected major compounds mangiferine and quercetine in Manadiar tablets and suspension, vitexin and chrysophanol in Manalaria tablets and suspension. HPLC data recoded for these analysed ameliorated medicines also indicated that their uniformity and homogeneity as already mentioned above, associated to the identification and quantification of these secondary metabolites led to considere these medicines as standardized.

Keywords: HPLC, fingerprintings, major compounds, identification, quantification, uniformity, homogeneity.