Abstract

OVERVIEW OF CAPILLARY ELECTROCHROMATOGRAPHY (CEC)

Bhumika Gidh*, Amey Deshpande, Shruti Sawant and Anurag Singh

ABSTRACT

Capillary electrochromatography (CEC) is a microscopic-scale separation technique that is a hybrid between capillary electrophoresis (CE) and liquid chromatography (LC). CEC can be performed in packed, monolithic and open-tubular columns. In the recent three years (from 2016 to 2018), much of the focus for CEC has been on the development of novel stable phases. In particular, some biomaterials attracted increasing interest. There has been no significant breakthrough in technology and principles at the CEC. Specific CEC applications, especially chiral separations, have been described. Capillary electrochromatography is a chromatographic technique in which the mobile phase is driven through a chromatographic bed by electroosmosis. Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. This review describes the general introduction to CEC, the various advantages of CEC over HPLC and Electophoresis, instrumentation, history, various stationary phase and mobile phases, column technology Advantages and applications of CEC in a variety of fields.

Keywords: Capillary electrochromatography, Electrophoresis, Technique, High performance liquid chromatography.