Abstract

STABILITY INDICATING ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CEFTAZIDIME AND AVIBACTAM IN BULK AND PHARMACEUTICAL DOSAGE FORM USING UPLC

Juaveria Salam* and Sh. Rizwan

ABSTRACT

This research work describes simple, sensitive, accurate, precise UPLC method for the simultaneous determination of Ceftazidime and Avibactam in pharmaceutical dosage form. The sample was analyzed by using C18 column (Waters Acquity C18 130A (100x2.2mm ID) 1.8μm with mobile phase using methanol, acetonitrile & water in the ratio of 60:20:20 in the flow rate of 0.5 ml/min. Detection wavelength was achieved at 248 nm. The retention time for Ceftazidime and Avibactam was found to be 2.365 and 4.460 minute respectively. The linearity for Ceftazidime and Avibactam was obtained in the concentration range of 100-300 μg/ml and 25-75 μg/ml respectively. Ceftazidime and Avibactam API and market formulation were subjected to acid and base hydrolysis, peroxide, thermal and photolytic forced degradation. In the forced degradation study Ceftazidime and Avibactam showed degradation in peroxide and acid degradation. The developed method was simple, specific, sensitive, rapid, and economic and can be used for estimation of Ceftazidime and Avibactam in bulk and pharmaceutical dosage form for routine analysis and stability studies.

Keywords: Cetazidime, Avibactam, UPLC method, Methodvalidation, Forced degradation.