Abstract

CYTOTOXIC POTENTIAL ASSESSMENT OF LINDERNIA CRUSTACEA (L.) F. MUELL EXTRACTS AGAINST HEPG-2 CELL LINES: AN IN-VITRO STUDY

S. Uma Krithika*, G. Suresh Kumar, K. Divya and K. Anitha

ABSTRACT

Hepatocellular carcinoma (HCC) is fatal in 80-90% cases because liver is the vital organ participates in various functions essential for life. The toxicity produced by chemotherapeutic drugs prescribed for liver cancer promotes the medicinal plant investigation in last decades. The literature survey fortified the traditional uses and distinct biological activities of Lindernia crustacea (L.) F. Muell including anti carcinogenicity The work was to explore the cytotoxic potential of Lindernia crustacea (L.) F. Muell against Human hepato cellular carcinoma (HepG-2) cell lines by in-vitro MTT assay method for first time. Ethanol, ethyl acetate and petroleum ether extracts of Lindernia crustacea (L.) F. Muell were prepared and subjected to anticancer evaluation. The crude petroleum ether, ethyl acetate and ethanol extracts were showed 91.24±1.2 %, 88.86±1.2% and 43.39±0.8% inhibition per 1000 μg/ml. As per the results, maximum anticarcinogenic effect against Hep-G cell lines was exhibited by petroleum ether extract followed by ethyl acetate extract. Ethanolic extract of Lindernia crustacea (L.) F. Muell was failed to show significant cytotoxic activity. The results were manifest the anti cancer activity of Lindernia crustacea (L.) F. Muell against HepG-2 cell line successfully.

Keywords: Hepatoprotective, Liver, MTT, Hepatocellular carcinoma, Lindernia crustacea.