Abstract

IN VITRO REGENERATION OF MINT (MENTHA SP.) AS AFFECTED BY DIFFERENT LEVELS OF PLANT GROWTH REGULATORS

Aaradhna Chauhan*, Sanjeev Lal and Mahendra Pal Singh Parmar

ABSTRACT

Mint is one of the most important traditionally used plant species as medicine and food. It contains essential oil, Menthol, which is used in many industries. In current study, the effect of various concentrations of plant growth regulators (BAP and NAA) was observed for in vitro establishment as well as in vitro rooting on Mentha Sp. Nodal explants were inoculated on MS media supplemented with auxin and cytokinin. Present work was carried out at Tissue Culture Lab, Department of Botany, R.C.U. Govt. (P.G.) College, Uttarkashi, India. Maximum bud response (67.5%) and number of shoots (3.5) was found in explants inoculated in MS media supplemented with BAP/ NAA at 1.5/0.5 mg/l concentration. For rooting, auxin IBA was used in half strength MS media at different concentrations. Maximum rooting (41.2%) was achieved with IBA 1.5 mg/l. This work will be helpful to increase the multiplication rate for micropropagation of Mentha sp.

Keywords: In vitro. Micropropagation. Mentha. Auxin. Cytokinin. MS media.