Abstract

IDENTIFICATION OF OVEREXPRESSED GENES IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA USING RNA-Seq METHOD

Preethi V.G, *Indira Priyadarsini R, and Kusum Paul.

ABSTRACT

Esophageal Squamous Cell Carcinoma (ESCC) is a type of cancer and may be caused due to the variation at the transcript level. Aberrant variations in splicing may lead to the progression of cancer. Identification of such splice forms can be used as a good biomolecular marker. With the advent of RNA-Seq method there has been a tremendous applications in discovery front and improvement in health care. The applications of RNA-Seq method are vast, including genome assembly to genome correction and its annotation in numerous organisms. RNA-Seq method analysis have discovered novel biomarkers for the early diagnosis of various diseases. However the expression of genes at transcript levels and identification of novel splice forms has not been done at a larger scale, which could be used to identify potential biomarkers for ESCC. Here ESCC sample datasets (one tumor dataset and one normal dataset) has been downloaded from public repository database (NCBI-SRA). 5 genes were found to be significantly overexpressed in tumor than in the normal sample. These genes can be used as biomolecular marker for the diagnosis of ESCC. Further experimental analysis has to be carried out in order to validate these 5 genes in order to prove those genes are upregulated.

Keywords: ESCC, Biomolecular marker, RNA-Seq method, splice variants.