Abstract

EFFICACY OF SELECTED HERBAL CHEWING PRODUCTS ON PREVALENT ORAL FLORA

Umeshwar Narayan* and Amar Prakash Garg

ABSTRACT

In vitro study, Aim was to study antimicrobial activities of selected chewing product against S. mutans and C. albicans. Three methods were used to obtain extract of chewing products in this study. Aqueous extraction method, Ethanolic extraction method and Soxhlet extraction methods were used. Chewing product was dried in shade condition. It was grinded in fine powder as per requirement and processed using water and ethanolic extraction method. Herbal crude extract were obtained. Concentrated by vaporization and herbal extract stock suspension were prepare in DMSO solvent. For antimicrobial potential, Ethanolic extraction method was observed better than water extraction method. Cultures of S.mutans and C.albicans were used in this study and were maintained in the department as pure culture. To asses, antimicrobial activities, fresh cultures were used. All testing pure culture were grown on Mitis-Salivarius Agar medium(MSA), Hi-Media Mumbai and Sabouraud’s Dextrose Agar medium (SDA), Hi-Media Mumbai (selective media for fungi containing chloramphenicol antibiotic) at 37°C for 24 h. Inoculums densities were prepared as compared to MacFarlane standard. 100 μl of test organisms were spreaded by using spread culture technique wait for a minute to absorb test organism suspension onto surface of Muller- Hinton agar medium. 6 mm of diameter wells were cut by using sterilized borer. 100 μl of testing herbal extract were filled in the wells. All petriplate were placed in Incubator at 37°C for 24h. Inhibitory zone were measured in mm. Broth Dilution Method (BMD) was used to detect MIC MBC and MFC of test microorganism. Gutka, cardamom and fennel seeds showed antimicrobial activities but extract of tobacco did not show antimicrobial activities. S. mutans showed inhibitory zones ranges from 14 to 2o mm and C.albicans, from 12 to 19 mm for extract used in these studies. Cardamom showed better MIC (6.25 mg /mL) and MBC (25 mg /mL) against S. mutans. MIC (3.125mg/mL) and MFC (12.5 mg/mL).

Keywords: Streptococci mutans, Candida albicans, and herbal extract.