Abstract

FORMULATION AND CHARACTERIZATION OF ALLIUM SATIVUM AND HONEY BASED HYDROGEL AND EXPLORING ANTIMICROBIAL EFFECT BY IN VITRO AND IN SILICO TECHNIQUES

Prajakta G. Bonde*, Priyanka S. Patil, Pranali C. Baviskar and R. S. Bachhav

ABSTRACT

Objective: The prime objective of this work is to develop a hydrogel formulation containing Allium sativum and honey as key ingredient and assess it by in vitro and in silico techniques to explore its antimicrobial activity. Material and Method: The hydrogel was formulated using homogenizationby incorporating carbopol 934, propylene glyco, and methyl and propyl parabens. The in vitro assessment of its antimicrobial activity was done against E. coli selecting gentamicin as control. The in silico activity was examined by docking of allicin with the E. coli DNA gyrase (PDB ID: 6RKS) by employing AutoDock Vina program and visualized by Discovey Studio and Pymol. Result: The formulation (F-4) was encountered to show high degree of in vitro antimicrobial activity in terms of zone of inhibition (18.48 mm) and prominent in silico DNA gyrase inhibitory activity of allicin was investigated with the affinity of -8.2 kcal/mol. Conclusion: The formulation was encountered to exhibit potential antimicrobial effect and since it is herbal based and offer greater palatability, it can be recognized as promising formulation for topical application to treat dermatological infections.

Keywords: Formulation, Gel, Antimicrobial, Solubility, Diffusion, Molecular docking.