Abstract

DEVELOPMENT AND EVALUATION OF ANTI -HYPERTENSIVE DRUG LOADED SOLID LIPID NANO- PARTICLES

Manojkumar G. N., Ashok Kumar P.* Manjunath K. and Girisha G. R.

ABSTRACT

The intention of the present study was to design Doxazosin Mesylate solid lipid Nano-particles and to evaluate them. Doxazosin Mesylate solid lipid Nano-particles were prepared by hot homogenization technique using different lipids (Tristearin, GMS and Comprital), soy lecithin as stabilizers and tween 80 as surfactant, evaluated for particle size & PDI, zeta potential, entrapment efficiency and in vitro drug release. The particle size ranged from 35.88 to 571.3 nm. PDI of all formulations were good within the range of 0.136 to 0.600. The zeta potential of blank SLN of TS was ranged from – 15.2 mV to -18.4 mV whereas drug loaded SLN with TS showed zeta potential from -21.0 to -24.7mV. Whereas drug loaded SLN with GMS and CM ranged from -2.03 to 42.0. Entrapment efficiency was in the range of 78.68 to 95.23 %. The cumulative percentage release of doxazosin Mesylate from different Doxazosin Mesylate Nano-particles varied from 55.74 to 89.58% depending upon the drug lipid ratio and the type of lipid used. The average percentage of drug released from different SLNs after 24 hours showed in the following order: F7 (55.74%) < F6 (57.18%) < F4 (61.56%) < F8 (65.85%) < F5 (65.87%)

Keywords: Doxazosin Mesylate, solid lipid Nano-particles, FTIR, in vitro drug release.