Abstract

ANTIFILARIAL POTENTIAL OF VITEX NEGUNDO L. LEAVES AND DIETHYLCARBAMAZINE CITRATE AGAINST SETARIA CERVI IN VITRO

*K.N. Sahare, and V. Singh

ABSTRACT

Herbal medicine is quite well known in Ayurveda and Siddha system of medicine. Hence the present study was designed to study the antifilarial effect of Vitex negundo L. leaves Methanolic extract and DEC against Setaria cervi in vitro. Activity was explored after 24 hrs incubation with concentrations ranging of 0.006 to 1.5 mg/ml for possible antifilarial effect, in terms of motility inhibition assay and MTT reduction assay. Vitex negundo L. Leaves showed significant antifillarial activity as compared to DEC was comparatively less significant in dose dependent manner. Inhibitory concentrations (50%) for with significant antifilarial activity in vitro system have been found to be 0.049 mg/ml for Methanol extract and for DEC it was 0.30 mg/ml calculated. The present research study shown significant antifilarial activity of Vitex negundo L. leaves Methanolic extracts highlights the further, isolation of active molecule from this plant for future drug designing.

Keywords: Anti-filarial, In-vitro, Drug designing, medicinal plant, DEC.