Abstract

ROLE OF PROTEIN OXIDATION AND LIPID PEROXIDATION MARKERS STATUS IN NIDDM PATIENTS

Kalaivanam. K N, Santhosh Kumar.N*, Bheemasen. R , Balu Mahendran.K

ABSTRACT

AIM & Objective: The link between hyperglycemia, enhanced free radical activity, and the complications of diabetes is unknown. The purpose of this study is to evaluate the levels of Protein Carbonyl, malondialdehyde (MDA) measured as thio-barbituric acid-reactive substances (TBARS), an index of protein oxidation, lipid peroxidation & microalbumin in patients of type 2 diabetes without complications compare with normal subjects of the same population. Methodology: We recruited 60 type 2 diabetic subjects without complications and with poor metabolic control and 60 age-matched controls with good metabolic control. Levels of Protein Carbonyl, glucose, total cholesterol, HbA1C, and MDA as TBARS, micro-albumins were determined. Results: Diabetic patients had higher levels of blood glucose (P<0.001), HbA1C ( P<0.001), Protein Carbonyl( P<0.001), microalbumin ( P<0.05)and MDA (P<0.001) than control subjects. The total cholesterol of the control subjects and diabetic patients did not differ. There was no correlation between the family history in diabetics and elevation in either HbA1C or MDA levels. Conclusion: To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), increased levels of Protein Carbonyl, MDA may be a useful marker of oxidative stress. The enhanced lipid peroxidation & protein oxidation leads to an increase in free-radical activity in type 2 diabetics. This increase in free-radical activity in type 2 diabetes mellitus along with insulin resistance can lead to activation of stress-sensitive pathways, which may play an important role in the complications of diabetes.

Keywords: Protein Carbonyl (PCO), malondialdehyde (MDA), type 2 diabetes, thiobarbituric acid-reactive substances (TBARS), HbA1C.