Abstract

A REVIEW STUDY ON SIMULTANEOUS DETERMINATION OF ABACAVIR, LAMIVUDINE AND ZIDOVUDINE BY USING DIFFERENT TYPES OF ANALYTICAL TECHNIQUES

N. Renuka Prabhandana*, M. Bala Krishna and K. Padma Latha

ABSTRACT

HIV(Human Immunodeficiency virus) is a virus attack the cells and body’s immune system that help the body fight infection, that makes the person more vulnerable to other infections and diseases. According to worldwide statistics there are 38.0 million people are living with HIV virus. The drugs abacavir, lamivudine and zidovudine combination drugs are used to treat and prevent HIV infection. Abacavir is mainly used to treat human immune deficiency virus (HIV). Abacavir works by decreasing the amount of HIV in blood of HIV patients. Lamivudine is the antiretroviral drug is used with other HIV medications which is helpful for the control of HIV infection. Lamivudine is used for the treatment of hepatitis B infection. Zidovudine is generally recommended for use with other antiretroviral drugs. Zidovudine is used to prevent mother-to-child spread during birth or after a needle sick injury or other potential exposure. So, combination of drugs such as abacavir, lamivudine and zidovudine has been developed, evaluated and validated according to ICH guidelines. The goal of this article is to develop different analytical methods for the quantitative and qualitative determination of abacavir, lamivudine and zidovudine. So, based on the ICH guidelines some of the analytical methods like RPHPLC, HPTLC, LCMS and UPLC methods are taken into consideration for method development and validation.

Keywords: Abacavir, lamivudine and zidovudine, high performance thin layer chromatography, high performance liquid chromatography, liquid chromatography mass spectroscopy and ultra performance liquid chromatography.