Abstract

STUDY OF THE RELATIONSHIP BETWEEN SEMINAL PLASMA HYPER VISCOSITY AND OXIDATIVE STRESS IN INFERTILE MEN

*Dr. Husham Salih Hammood

ABSTRACT

Objective To show the relationship between semen hyper viscosity and oxidative stress in infertile male by measuring MDA as a biomarker of oxidative stress and PON-1 as an antioxidant agent in hyper viscid semen. Patients And Methods: Hyper viscid Semen was collected from 60 infertile patients who attended to outpatient clinic of andrology department in Kasr AL-Ainy hospitals; the samples were subjected to semen fluid analysis according to WHO criteria of 2010 to determine the viscosity and other parameters of semen. Then the samples were centrifuged and seminal plasma was separated and Freezed in a temperature -70 c without preservative for one or two months and then determine the level of oxidative stress in form of malondialdehyde (MDA) and anti-oxidant capacity in form of paraxonase-1 (PON-1). The patients were selected after exclusion criteria such as lukocytospermia, smoking, alcohol intake, chronic diseases and anti-oxidant therapy. Results  The level of MDA showed a highly significant difference between the Azoospermic and Normozoospermic groups (p = 0.006), and again between the Normozoospermic and Oligo and/or Asthenozoospermic groups (p

Keywords: Semen hyper viscosity, oxidative stress (OS), anti-oxidant (AO), Azoospermia, Normozoospermia, Oligo and /or Asthenozoospermia, Malondialdehyde (MDA), Paraxonase-1 (PON-1).