Abstract

STUDY OF EXTENSIVE COLD EXTRACELLULAR ALKALINE PROTEASE PRODUCED BY PSYCHROTROPHIC Stenotrophomonas maltophilia STRAIN BBM7

Syed Nisar A.*, Syeda Kulsoom F. and Mohammed Shakir

ABSTRACT

A novel psychrotolerant bacterium Stenotrophomonas maltophilia, was isolated with the ability to produce extracellular, extensive cold, alkaline, and detergent-stable protease from refrigerated milk obtained from dairy of Aurangabad. In terms of incubation time, agitation, substrate, pH, and temperature, the culture conditions for higher protease production were optimized. After 5 days of incubation, a maximum protease production of 54 U mL–1 was achieved in the medium at 200C and pH 9.0. The protease was found to have excellent stability and consistency with commercial detergents, as well as a high efficiency for extracting various protein-containing stains at low temperatures. In contrast to detergent alone, the wash efficiency study of blood and soil stains on cotton fabric revealed a 20% improvement in reflectance after enzyme therapy. These findings mean that it may be a viable factor for use as a cold-washing detergent additive and in cold-climate environmental bioremediation.

Keywords: Psychrotolerant, Stenotrophomonas maltophilia, alkaline protease, bio detergent, extensive cold enzyme.