Abstract

MORPHOLOGICAL AND HISTOLOGICAL STUDY OF ALANGIUM SALVIFOLIUM AND PHYTOCHEMICAL SCREENING OF ITS EXTRACT

Aniket M. Ingole*, Renuka J. Das, Milind J. Umekar

ABSTRACT

The present study was aimed to carry out preliminary phytochemical screening, thin layer chromatography and antimicrobial study of petroleum ether and ethyl acetate extract of Alangium salvifolium leaves. The plant has been reported to be used traditionally for the treatment of purgative, anticonvulsants, astringent, antiulcer, pungent, alleviates spasms, anthelmintic, emetic, antiprotozoal, hypoglycemic agent and helicon bactericidal activity. The plant material was collected, leaves were sun dried and pulverized into coarse powder. It was then extracted with petroleum ether and ethyl acetate by soxhlation. The extract was subjected to preliminary phytochemical screening to detect the presence of primary and secondary metabolites. It showed the presence of alkaloid, glycosides, terpenoids, steroids, tannin and flavonoids. Different solvent systems were tried for developing the mobile phase to obtain TLC profile of petroleum ether and ethyl acetate extracts of Alangium Salvifolium leaves and the better separation of the components was observed in toluene: ethyl acetate (10:1) solvent system. Two components were found to be separated in petroleum ether extract with corresponding Rf values 0.8 and 0.9 And 3 components were found to separated in ethyl acetate extract with corresponding Rf values 0.5, 0.8 and 0.9. Both extract also give the positive antimicrobial effect. Further studies on the mechanism of actions of this plant and identification of the constituent compounds responsible for the pharmacological activities should be carried out in future.

Keywords: Alangium salvifolium, petroleum ether, Ethyl acetate, TLC, Phytochemical screening, antimicrobial study.