Abstract

AS IMMUNOADJUVANT SYSTEMS INIVACCINE FORMULATIONS: FOR PROTEIN CARRIER

*Pooja Bharti and Neha Soni

ABSTRACT

The immunoadjuvant potential of Asparagus racemosus (Willd) Family (Liliaceae) aqueous root extract was evaluated in experimental animals immunized with diphtheria, tetanus, pertussis (D TP) vaccine. Immunostimulation was evaluated using serological and hematological parameters. Oral administration of test material at 100 mg/kg per day dose for 15 days resulted significant increase (P = 0.0052) in antibody titers to Bordtella pertussis as compared to untreated (control) animals. Immunized animals (treated and untreated) were challenged with B. pertussis 18323 strain and the animals were observed fori14 days. Results indicate that the treated animals did show significant increase in antibody titers as compared to untreated animals after challenge (P = 0. 002). Immunoprotection against intra-cerebral challenge of live B. pertussis ells was aluated based on degree of I sickness, paralysis and subsequent death. Reduced mortality accompanied with overall improved health status was observed in treated animals after intra-cerebrachallenge of I B. Pertussis indicating development of I protective immune response. Present study indicates applications of test material as potential immunoadjuvant that also offers direct therapeutic benefits resulting in less morbidity and mortality.

Keywords: Exosome; Immune checkpoint inhibitor; Immunoadjuvant; Phototherapy; Toll- like receptor.