Abstract

PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF CARISSA CARANDAS LEAVES

Milan Gautam*, Dipanshu Panday Amber Mishra and Rajvinder Kaur

ABSTRACT

Medicinal plant have been used for centuries as a remedy for human disease because they contain components of therapeutic worth. The acceptance of conventional medicine as an a alternative type health care and development. The present research confined to the study of phytochemical screening and anti-oxidant activity of Carissa carandas leaves. The leaves were collected from Carissa carandas tree dried under the sheds and made fine coarse powder. The powder was poured in 1 liters of petroleum ether for 48hrs, it is important for removal of fatty acid and oils. Then the coarse powder was successively extracted with Distilled water (extract 1) and Ethanol: Water (1:1) (extract 2) by soxhlation (Soxhelt apparatus). Various chemical test of leaves showed that glycoside, flavonoids, tannins, terpenoids, alkaloids, carbohydrates, phenols, amino acid and saponins. The phytochemical creening of extract was done by column chromatography, characterization of compound were done on the basis of U.V., I.R., and N.M.R. spectral data. The antioxidant potential of plant Carissa carandus leaves extracted with different polarity base solvents was assessed. The antioxidant activity (in vitro) was evaluated by measurement DPPH scavenging activity method.

Keywords: Anti – Oxidant Activity, Anti – Bacterial Activity, Hepato – Protective Activity, Anti – Inflammatory Activity.