Abstract

THE EVALUATION AND COMPARISON OF RADIOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACTS OF CAPPARIS APHYLLA AND PAEDERIA FOETIDA ON SWISS ALBINO MICE AGAINST WHOLE BODY ELECTRON BEAM IRRADIATION

Shastry CS*, Aswathanarayana BJ, Ganesh S, Kalluraya B, Maurya DK

ABSTRACT

The present study was aimed at the evaluation and comparison of radioprotective activity of 200 and 400 mg/kg dose of ethanolic extracts of Capparis aphylla and Paederia foetida. The study consists of in-vivo 30 day survival study against whole body sub lethal dose, 6 Gray (Gy) of electron beam radiation on Swiss albino mice which was followed by various biochemical assays. In in-vitro study, radiation exposure to mitochondrial fractions with and without extracts along with free-radical scavenging activities was carried out. The five days pretreatment of mice with Capparis aphylla and Paederia foetida delayed the onset of mortality and increases the survival rate. Capparis aphylla shows highest survival rate of 40% than Paederia foetida. The both extract treated groups shows decrease in lipid peroxidation activity and increase in glutathione-S-transferase (GST), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSHPx) & glutathione reductase (GR) activities compared to irradiation control group significantly. The radiation exposure to mitochondrial fractions of rat liver with both extracts shows reduction in lipid peroxidation activity which shows decrease in oxidative stress. Both the extracts show a concentration dependent in-vitro freeradical scavenging in DPPH, ABTS, Total Antioxidant Capacity and H2O2 activities. The finding of the present study provides the evidence that, ethanolic extracts of Capparis aphylla and Paederia foetida shows a prominent radioprotective activity by antioxidant and free-radical scavenging mechanism. Capparis aphylla was found to be better radioprotector than Paederia foetida through in-vivo and in-vitro models

Keywords: Capparis aphylla, Paederia foetida, Electron beam radiation, radioprotective, antioxidant, free-radical scavenging.