Abstract

APPLICATIONS AND QUANTITATION OF HILIC

Ashok E. Rathod*, Ritesh R. Ojha and Dr. Purnima Hamrapurkar

ABSTRACT

Hydrophilic interaction chromatography (HILIC) is a mode of liquid chromatography that provides effective separation and retention of highly moderate hydrophilic and polar compounds. HILIC are based on the use of hydrophilic stationary phases routinely exploited by normal-phase liquid chromatography (NPLC) combined with mobile phases (aqueous /polar organic solvent mixtures) typically used into reversed-phase liquid chromatography (RPLC)., It accepted the HILIC retention and separation of ionic and ionizable compounds which, traditionally, require separation by ion exchange chromatography (IC) and ion pairing reversed-phase chromatography (IP-RPLC). Retention mechanism in the HILIC results from a combination of analyte partition between the aqueous layer associated with the hydrophilic surface of the stationary phase and the organic component of the mobile phase, with other type of interactions are the hydrogen bonding, dipole-dipole, electrostatic and even hydrophobic interactions. Mechanisms that contribute to HILIC separation are explored1,2 with the aim of achieving a better understanding of analyte retention and selectivity and thus facilitating the development of new methodologies under HILIC conditions. Not only the use of the appropriate stationary phase may play of main role in the optimization of a HILIC method, but also the composition of the mobile phase and the nature of the analytes should be considered in obtaining a better separation.

Keywords: Hilic, Nplc, Rplc, Ip-Rplc, Hplc, Stationary Phase, Mobile Phase.