Abstract

DETECTION OF TOTAL POLYPHENOL, GALLIC ACID AND VINCA ALKALOIDS IN CATHARANTHUS ROSEUS ORIGINATED FROM BINH THUAN PROVINCE (VIETNAM)

Nguyen Pham Quynh Anh, Doan Thi Thanh Vinh, Vo Duy Le Giang and Nguyen Hoang Khue Tu*

ABSTRACT

Catharanthus roseus, commonly known as periwinkle or annual vinca, is available in a range of shades including, white, pink, red and purple. This species produces a variety of secondary metabolites. Hence, the vincristine and vinblastine contents in those plants are also different. Besides, the contents of these compounds are so low that finding a substitute resource to produce a large amount of vincristine and vinblastine is necessary. This study aimed to collect periwinkles with pink flowers from Binh Thuan province (Vietnam) and detect polyphenols, vincristine and vinblastine derived from plants. The extracted solutions were detected for polyphenols by Folin Ciocalteu (FC) method. The essential compounds such as gallic acid, vincristine and vinblastine were tested for presence by high performance liquid chromatography (HPLC) analysis. The results showed that the extract had polyphenols with a concentration of 3.53 ± 0.05 mg GAE/ml. On chromatogram, gallic acid, vincristine and vinblastine had retention time of 2.874 minutes, 3.6 minutes and 7.683 minutes as the same as retention time of standard gallic acid, vincristine and vinblastine, respectively. The study concluded that collected Catharanthus roseus plant had biological compounds for pharmaceutical field.

Keywords: Gallic acid, vinblastine, vincristine, polyphenol, HPLC, Catharanthus roseus.