Abstract

SYNERGISTIC ANTI-INFLAMMATORY ACTIVITY OF DOLICHOS BIFLORUS STANDARDIZED SEEDS EXTRACT AND CICHORIUM INTYBUS STANDARDIZED ROOTS EXTRACT COMBINATION

Anupama Yadav*

ABSTRACT

After exploring numerous pharmaceutical databases such as PubMed, Google Scholar, Scopus, etc., it was observed that no combined anti-inflammatory perspectives of Dolichos biflorus seed extract with Cichorium intybus root extract in an in vitro method (human red blood cells, HRBC) have been reported yet. Therefore, it was planned to screen the D. biflorus standardized seed extract (DBSSE) and C. intybus standardized root extract (CISRE) together to explore the synergistic activity. At a concentration of 250 μg/mL, DBSSE provided 34.51% membrane stabilization/protection in HRBCs and CISRE provided 41.19% membrane stabilization/protection in HRBCs. When the extracts DBSSE and CISRE were tested together (at a concentration of 250 μg/mL each), the anti-inflammatory effect was increased to 62.15%, quite synergistically. There was a 20% to 25% increase in the anti-inflammatory benefit when both the extracts were used simultaneously. The phytoconstituents found in plant material, specifically flavonoids, phenols, and alkaloids, play a key role in mediating anti-inflammatory action by inhibiting various inflammatory enzymes. The extract with high levels of phenolic and flavonoid content stabilized the HRBC membrane in a synergistic manner. This report will open up new avenues for potential research in the development of innovative herbal formulations for acute or chronic inflammation treatment.

Keywords: Dolichos biflorus, Cichorium intybus, Extract, Anti-inflammatory, HRBC Method, Phytoconstituents.