Abstract

EVALUATION OF ANTICANCER POTENTIAL OF PAJANELIA LONGIFOLIA (WILLD.) K. SCHUM. BY MTT ASSAY METHOD

Gini E. J.*, Anju Mathew, Anju Paul, Athira Jaimon and Sreekeerthana K. S.

ABSTRACT

Colorectal cancer is one of the most common cancer, both in men and women and in United states it is the most common cause of death. When compared to synthetic drugs herbal medicines are less toxic and are widely used in cancer therapy. Pajanelia longifolia (Willd.) K. Schum., belong to the family Bignoniaceae, is commonly distributed in the Western Ghats and Eastern Bengal in India preliminary phytochemical evaluation of Pajanelia longifolia showed the presence of flavonoids, terpinoids, tannins and phenolic compounds. Polyphenols have a major role in colorectal cancer prevention. Hence in this study an attempt was made to evaluate the in-vitro anticancer potential of aqueous and alcoholic extracts of Pajanelia longifolia (Willd.) K. Schum by standard MTT assay using DLD -1 cell lines. Both the extarcts possess anticancer activity in a dose dependent manner, maximum activity showed at 400μg/ml. IC 50 for aqueous and alcoholic extracts was found to be 362.5μg/ml 252.5μg/ml respectively. Among the extracts, ethanolic extract showed good anticancer potential against DLD-1 cell lines. From this study it can be concluded that both the extracts of Pajanelia longifolia possess anticancer activity against DLD -1 cell lines in a concentration dependent manner.

Keywords: Pajanelia longifolia (Willd.) K. Schum., Soxhlet extraction, MTT assay method.