Abstract

HEPATIC INJURIES INDUCED BY PHENTOLEP DRUG AND HEPATIC REGENERATIVE CAPASITY IN MALE ALBINO MICE Mus musculus: MOLECULAR AND HISTOLOGICAL STUDIES

Nagla Zaky Ibrahim El -Alfy* and Amany Ibrahim Alqosaibi

ABSTRACT

Present investigation was carried out to evaluate the hepatic regenerative capacity after the treatment with commonly clinical prescribed phentolep drug through applying effective complementary parameters. Comet assay was carried out on hepatocytes to measure the DNA damages and histological study to assess hepatic injuries. Present experiment was carried out on 4 groups of male albino mice Mus musculus. Frist group was negative control injected with physiological saline (1ml/kg). Group 2 was positive control injected with phentolep drug (12mg/kg) for 2weeks, group 3 injected with phentolep drug (12mg/kg) for 2weeks then allowed to recovery for 2 weeks and group 4 injected with phentolep drug (12mg/kg) for 2 weeks then cared for 4 weeks without treatment. Results indicated that treatment with phentolep drug (12mg/kg) for 2weeks caused high significant level of damage in DNA content of hepatocytes nuclei and hepatic histopathological changes where groups that were cared for 2 and 4 weeks displayed variable degrees of regression in the level of DNA damage and restored the normal histological pattern of liver depend on time with reference to negative control group. In current experiment hepatic injuries were reversible with time dependency.

Keywords: DNA, epilepsy, liver, phenytoin.