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METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF TIMOLOL MALEATE AND TRAVOPROST IN BULK AND IN PHARMACEUTICAL DOSAGE FORM BY UV-SPECTROSCOPY AND RP-HPLC
Jothieswari Dhamotharan*, Kovai Venkata Sai Heera, Amasa Pavan Kumar, Katta Govardhan, Kadiyala Himaja, Siva Kumar Vidya Sagar and Bala Venkata Ramana Chandana
ABSTRACT
The blend of these two medications (Timolol maleate and Travoprost) is utilized to bring down the weight in the eye when one of the drugs doesn't bring down the weight enough all alone. The eye drops should be layed into the influenced eye(s) once every day. The point of the current examination is to grow new scientific strategies for the synchronous assessment of Timolol maleate and Travoprost in mass and in Pharmaceutical dose structure. Spectroscopic strategies have been produced for the evaluation of Timolol maleate and Travoprost in mass and in the plan. A straightforward, fast and exact RP-HPLC strategy for synchronous quantitative assurance of Travoprost and Timolol maleate in ophthalmic arrangement was created. Chromatographic partition was accomplished with PDA finder utilizing PHENOMENOX C18, 15O×4.6 mm; 5 micron turn around stage logical segment. The portable stage comprise of ortho phosphoric corrosive (0.1%): methanol (60: 40 v/v), was gone through the segment at stream pace of 0.8 ml/min. The strategy was performed at the frequency 272nm by isocratic procedure. The investigation was done at 25oC. The maintenance season of Travoprost and timolol maleate was discovered to be 6.322 min and 3.730 min. The alignment bends were direct in the focus scope of half to 150% of the working fixation (r2> 0.999). The lower furthest reaches of evaluation were 0.142 and 0.0069 for Timolol maleate and Travoprost individually. The lower furthest reaches of location were 0.043 and 0.0021 for Timolol maleate and Travoprost respectively. The created strategy was utilized for synchronous quantitative assessment of Travoprost and Timolol maleate in ophthalmic arrangement. Created technique was approved according to ICH Q2 (R1), and it is generally valuable for scholastic just as mechanical scale. Keywords: Timolol maleate, Travoprost, Double distilled water, RP-HPLC method. [Download Article] [Download Certifiate] |