Abstract

CHARACTERIZATION OF LINUM USITATISSIMUMAND SESAMUM INDICUM SEED LECTIN AND ITS ANTIBACTERIAL ACTIVITY AGAINST SELECTED ORAL PATHOGENS

Dr. M. Krishnaveni*, J.Kowsalya and S. Maheswar

ABSTRACT

Objective: The main objective of the study is to isolate lectin from natural source (Linum usitatissimum and Sesamum indicum seed) and to characterize it for its biochemical properties as well as to test its antimicrobial potential especially on few selected oral pathogens. Methods: The isolated lectin was characterized for its carbohydrate, protein content qualitatively and quantitatively and also protein profile, hemeagglutination, hemeagglutination inhibition assay, effect of pH, temperature, salt, denaturing agent, SDS PAGE, anti- inflammatory, antioxidant, antibacterialactivity against Streptococcus oralis, Strptococcus pyogens, Propioni bacterium acnes, Staphylococcus aureus, Minimal inhibitory concentration was also studied. Results: The presence of carbohydrate and protein was observed. The results of protein profile was good for both the samples studied. The anti-inflammatory activity was higher for Linum usitatissimum seed. Positive result was obtained with O+,B+ blood groups and among the animals goat in hemeagglutination assay. Both showed positive results for glucose, sucrose, maltose in hemeaggluination inhibition assay. Similarly, both were found to be pH stable and temperature stability was noticed only with Sesamum indicum seed lectin. While, Linum usitatissimum seed lectin showed stability to denaturing agent urea. Positive result was obtained with FeCl3, Na2So4,CaCl2 for both the lectins.DPPH antioxidant activityshowed promising results. The band observed in SDS PAGE was found to be low in molecular weight. The antibacterial activity observed was good with Streptococcus oralis, Strptococcuspyogens, only mild activity was observed with Propioni bacterium acnes, Staphylococcus aureus. The results demonstrate that both the seed samples were able to exhibit positive result for the studied parameters confirming the presence of lectin.

Keywords: Anti-inflammatory, Antioxidant, Antibacterial activity, Thermo stable lectin, SDS PAGE.