Abstract

FORMULATION AND EVALUATION OF LAWSONIA INERMIS (SUDANESE HENNA) LEAVES EXTRACT AS SEMISOLID DOSAGE FORM FOR ANTIBACTERIAL ACTIVITY

Khawla Gamal Eddeen Ahmed Mohammed*, Alzahraa Mohamed Mohamed Salih, Ghofran Salah Ahmed Hamdoon, Hana Abd el Mahmoud el Tayeb and Abdelkarim M. Abdel Karim

ABSTRACT

Aim of the study: The present study was undertaken to extract, formulate and evaluate the antimicrobial activity of the Lawsonia inermis leaves extract and ointment. Methods: Lawsonia inermis leaves were collected from Al-thowra, Khartoum, Sudan during April 2016, and identified in Sudanese national center for research. The collected amount was dried at room temperature under shade for 3 days. Lawsonia inermis dried leaves were milled by porcelain mortar and pestle into small particles. 550g of the powder were extracted with 1900ml of ethanol by soxhlet apparatus at 70°C for 72 hours, the extract was phytochmically screened and tested for the antimicrobial activity. The extract was then dried, weighed and formulated as ointment dosage form and evaluated. Results: phytochemical screening of Lawsonia inermis leaves revealed presence of saponins, triterpenes, flavonoids, tannins, deoxy sugars and anthraquinone glycosides, alkaloids, phyto-sterols, comarine, proteins and amino acids. The extract was found to be active against gram positive Bacillus cereus and Staphylococcus aurous, and gram negative: Escherichia coli. The physicochemical test results of the prepared ointment complied with the compendia requirements of viscosity, pH, spreadability and content uniformity. Conclusion: It can be concluded that Lawsonia inermis leaves ethanolic extract was found to contain several active phytochemical compounds as alkaloids, saponins, phytosterol and anthraquinone glycosides, that are effective for many types of bacterial diseases.

Keywords: Lawsonia inermis, ethanolic extract, phytochemical screening, Ointment, antimicrobial activity.