Abstract

EVALUATION OF INVITRO ANTIOXIDANT ACTIVITIES OF VARIOUS EXTRACT FROM SEED KERNELS OF CAESALPINIA BONDUCELLA LINN.

Annadurai Thenmozhi, Cyril Jane Shammah* and Narayanan Venkateshan

ABSTRACT

The study was designed to examine the in vitro antioxidant activities of pet. ether, ethyl acetate and ethanolic extract of seed kernels of Caesalpinia bonducella Linn. The seeds were broken and kernel from outer seed shell was separated. Seed kernel of Caesalpinia bonducella was crushed by a mechanical processor and was extracted with pet.ether, ethyl acetate and ethanol by continuous hot percolation method using Soxhlet apparatus. The antioxidant activity was determined by DPPH assay, Superoxide anion scavenging activity, iron chelating activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, FRAP assay, total antioxidant activity (phosphomolybdic acid method) at four different doses 125 to 1000 μg/ml with reference natural standard rutin, quercetin, EDTA, Ascorbate respectively and total phenolic and total flavonoid content was analysed. An IC50 value was found that ethanolic extract of Caesalpinia bonducella is more effective. These in-vitro assays indicate that this plant extract are better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.

Keywords: Antioxidant, Caesalpinia bonducella, In-vitro antioxidant, Total flavonoid content, Total phenolic content.