Abstract

FORMULATION AND EVALUATION OF HERBAL ANTI-INFLAMMATORY GEL CONTAINING DAUCUS CAROTA LINN EXTRACT

Anita R. Desai, Supriya V. Hiremath*, Prabhu K. Halakatti and Y. Srinivas

ABSTRACT

The aim of the present work was to formulate and evaluate herbal anti-inflammatory gel containing Anthocyanin extract. Anthocyanin was extracted from the Daucus carota l. using 1% HCl (v/v) in methanol as a solvent. Anthocyanin gels were prepared using various polymers like Xanthan gum, Carbopol 934, HPMC K100 M and other additives. All the Anthocyanin gel formulations were evaluated for FTIR, pH, viscosity, spreadability, extrudability, drug content, in-vitro drug release. And results showed that the FTIR spectral analysis suggested that there were no interaction between the drug and formulation additives. pH of formulations were fairly constant about 6.1-6.7 within the range of skin pH. Gels prepared using xanthan gum were less viscous, more spreadability, excellent extrudability and showed better % drug content compared to gels prepared with Carbopol-934 and HPMC K100 M. Best formulations in terms of cumulative % drug release were formulations F1 and F2 with 90.3% and 87.7% respectively at the end of 6 hours. Hence F1 and F2 gels were further studied for In-vivo anti-inflammatory activity by rat paw edema method, gel F1 proved the best results in inhibiting the edema when compared to diclofenac gel.

Keywords: Anthocyanin, Anti-inflammatory, Herbal gel