Abstract

ISOLATION, PURIFICATION AND CHARACTERIZATION OF SWEETNERS FROM STEVIA REBAUDIANA (BERTONI) FOR THEIR ANTICANCEROUS ACTIVITY AGAINST COLON CANCER

Sarika R. Deshmukh* and Varsha R. Kedari

ABSTRACT

Stevioside, an abundant component of Stevia rebaudiana leaf, hasbecome well-known for its intense sweetness and is used as a noncaloricsweetener beside sweetness, it may also offer therapeuticbenefits. It is natural sweetner and contains phytochemicals includingwell characterized glycosides such as Stevioside, Reboudioside A-Fand Steviol. The present study was deal with extraction of Steviosidesand its derivatives from Stevia rebaudiana which is followed by itsidentification, analysis of its quality, quantity and biological activity.Stevioside was extracted from the dried Stevia leaves. Further it waspuified, recovered using Calcium hydoxide and Ion exchangechromatography. In fresh leaves extract the percentage of carbohydrates is higher thanreducing sugars. TLC was carried out for qualitative analysis. Confirmatory test foridentification and presence of Stevioside was positive. Steviosides have excellent heatstability which is up to 100°C for 1 h at pH range 3 -9.The HPLC analysis of water extracthad shown the presence of Rebaudioside-D, Stevioside and Rebussoside.Methanolic leafextract had shown the presence of Rebaudioside D and rebausdioside. Etahnolic extract hadshown a type of peak which was remained unidentified. Along with this, it showed presenceof Rebaudioside A and Rebussoside. The data obtained by 1H NMR showed isolatedcompound was Stevioside. The methanol and ethanol extract of Stevioside showed anticanceractivity against Caco cell line with IC50 value 10 and 12μg/ml respectively whereas theseextract had shown the cytotoxicity against Caski cell line with IC50 value of 20 and 5μg/mlrespectively.

Keywords: Stevioside, TLC, HPLC, 1H NMR, anticancer activity, LDH activity.