Abstract

EVALUATION & COMPARISON OF ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF AEGLE MARMELOS (BILWA) LEAF AND FRUIT WITH 0.2% CHLORHEXIDINE ON PERIODONTOPATHIC BACTERIA– AN IN-VITRO STUDY

Deepa V., Gayathri G. V., Vinayaka A. M.* and D. S. Mehta

ABSTRACT

Introduction: The present study was carried out with the aim of assessment of Minimum inhibitory concentrations (MIC) and Minimum bactericidal concentration (MBC) and time kill curve (TKC) analysis of ethanolic extract of Aegle Marmelos leaf and fruit on Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Fusobacteria nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa) and compare it with the MIC, MBC and TKC analysis of 0.2% chlorhexidine mouthwash. Materials & Methodology: MIC was carried out by using serial dilution method and cultures of microorganisms were incubated with the same drug concentrations to assess MBC. Time kill curve was analyzed by incubating the organisms in an anaerobic chamber, to evaluate the timely declination of their growth rate. Results: MIC values ranged from 25-100 μg/ml for tested microorganisms. MBC values showed that Aa and Pg were found to be highly susceptible to ethanolic extract of leaf (50 μg/ml) and Fn for fruit extract (50μg/ml) of Aegle marmelos for tested range of dilution. On time kill curve analysis all tested microorganisms were susceptible to the lowest tested dilution (0.2 μg /ml) and showed bacteriostatic but no bactericidal activity upto 2 hrs. Conclusion: Thus it can be concluded that, the ethanolic extract of Aegle marmelos leaf and fruit have showed antimicrobial efficacy against all the tested microorganisms and further invivo research to be carried out for the treatment of chronic periodontitis.

Keywords: Aegle marmelos; Antibacterial activity; Minimum Inhibitory Concentration; Minimum Bactericidal Concentration; Time kill curve.