Abstract

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF RESVERATROL AND PIPERINE IN COMBINED CAPSULE DOSAGE FORM

Jaldip Jasoliya*, Aashka Jani

ABSTRACT

A simple, precise, cost effective RP-HPLC method has been developed and validated for the determination of Resveratrol and Piperine in pharmaceutical compositions. The chromatographic separation was achieved on hibarR 250-4, C-18 columns (250mm ×4.6mm,5um) using a mobile phase consisting of Acetonitrile+water(95+5) : Methanol (60:40v/v) with pH 4.0 adjusted with Acetic acid at a flow rate of 1ml/min. Detection wavelength was found 305 and 336 nm for Resveratrol and Piperine respectively. The Retention times of Resveratrol and Piperine were found 2.808 and 3.508minute respectively. The method was found to be linear over the range of 10- 50 μg/ml and 1-5 μg/mlwith correlation co-efficient (r2) 0.999 & 0.999 for Resveratrol and Piperine respectively. Percentage recoveries obtained for both the drugs were 99.49-100.25% and 99.52-100.53% for Resveratrol and Piperine respectively. The %RSD for precision and accuracy of the method was found to be less than 2%.The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Resveratrol and Piperine from their combined dosage form.

Keywords: Resveratrol, Piperine, ICH guideline, simultaneous determination, RP-HPLC method.