Abstract

ANTIOXIDANT EFFECT OF A HERBAL FORMULATION IN PARACETAMOL INDUCED TOXICITY IN ALBINO RATS

Yuvarani S, Radhika J*, Jothi G, Sangeetha.D

ABSTRACT

Oxidative damage is implicated in the pathogenesis of a number of diseases. There are positive links between accumulated free radicals and drug induced toxicity. The interest in screening the antioxidant potential of plant-derived compounds to reduce the risk of diseases is increasing globally. The present study was designed to analyse the antioxidant potential of a herbal formulation comprising of Atrocarpus heterophyllus Lam (leaves), Curcuma amada Roxb (Root) and Piper longum Linn (fruits) in paracetamol induced toxicity. Wistar strains of Albino rats were used as the experimental models. The rats were divided into six groups comprising of six rats each. Group I served as a normal control. Group II was induced with paracetamol at a dose of 2g/kg bw as a single dose. Group III was induced with paracetamol and orally administered the formulation at a dose of 100mg/kg bw for 15 days. Group IV was induced with paracetamol and orally administerd the formulation at a dose of 200mg/kg bw for 15 days. Group V was induced with paracetamol and administered Liv – 52 at a dose of 3ml/kgbw/day for 15 days. Group VI received the formulation alone at a dose of 200/kg bw for 15 days. The effects of the plant extract on lipid peroxidation (LPO), enzymatic antioxidant – Superoxide dismutase (SOD), Non enzymatic antioxidant – Reduced glutathione (GSH) and protein (tissue and serum) were analysed. The disease control group showed a marked elevation in the LPO levels and a decrease in the protein levels and antioxidant status, which was reversed to near normal in the plant and Liv 52 treated groups. The present study suggests the antioxidant potential of the herbal formulation.

Keywords: Paracetamol, lipid peoxidation, superoxide dismutase, reduced glutathione, Antioxidant.