Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES USING FLOWER EXTRACTS OF CATHARANTHUS ROSEUS AND EVALUATION OF ITS ANTIBACTERIAL EFFICACY

Manisha D.R, Jahnavi Alwala, Karunakar Rao Kudle, M.P.Pratap Rudra*

ABSTRACT

Synthesis of metal nanoparticles involve wet chemical reactions and sometimes the used chemicals could be ignitable or toxic. Hence, there is much scope and environmental friendliness in synthesis of metal nanoparticles by using biological material. In the present study, the synthesis of stable silver nanoparticles by the bioreduction method have been discussed. Silver nanoparticles are formed by incubating aqueous flower extract of the plant Catharanthus roseus with aqueous solution of silver nitrate (AgNO3). In this the plant extracts act as the reducing agent for the reduction of Ag+ to Ag0. A change in color from pale yellow to reddish brown and a strong peak at 460nm under UV spectral analysis confirmed the formation of silver nanoparticles. FTIR analysis indicated the functional groups responsible for the formation of AgNPs. Results obtained from Transmission Electron Microscopic studies indicated that the formed silver particles were within the range 6-25nm. The antibacterial activities of the formed silver nanoparticles have also been discussed.

Keywords: Catharanthus roseus flower extracts, bioreduction, TEM and Anti bacterial activity.