Abstract

TOXICITY PROFILE OF ETHANOLIC EXTRACT OF LAWSONIA INERMIS LEAVES IN ALBINO WISTAR RATS

Manpreet Kaur, CBS Dangi, Ashish Singhai, Madulika Singh, Susmit Kosta,Hardev Singh, Jaya Peter, Shikha Jain

ABSTRACT

Leaves extract of Lawsonia Inermis (Heena) is extensively used for medicinal purpose in various countries. The aim of the present study is to determine the adverse effects of leaves extract of L.Inermis using albino wistar rats of both sex. Acute toxicity profile was determined with dose pattern of 100mg, 500mg, 1000mg and 2000mg /kg/b.w for 72 hours. After determining the lethal dose sub-acute study was study was conducted with dose 200mg, 500mg and 1000mg /kg/b.w. for 14 days . After 14 days blood was taken for various biochemical test and animals were scarified, heart, liver, kidney and spleen were histologically studied. The histopathological and biochemical results showed that no pathological and haematological changes were observed with dose level of 200 and 500mg/kg/b.w. when compared with the control group.

Keywords: Acute toxicity , Biochemical, Heena, Histopatholical, Lawsonia interminis, Sub-acute.