Abstract

CHEMICAL PROFILE AND BIOLOGICAL ACTIVITIES OF THE ETHANOLIC EXTRACT OF EUGENIA PRUNIFORMIS CAMBESS (MYRTACEAE)

Fernanda Leite Pereira Martins*, Tayane Fardim Correa, Rodrigo Rezende Kitagawa, Luís Fernando Tavares de Menezes, Álvaro Cunha Neto, Ricardo Machado Kuster, Damaris Silveira and Claudia Masrouah Jamal

ABSTRACT

Eugenia pruniformis Cambess (Myrtaceae) is popularly called “azeitoninha da praia”, is an endemic species from Brazilian Atlantic Forest. The study aimed to evaluate the chemical profile of ethanol extract of leaves from E. pruniformis (EEEP), and two in vitro activities: antioxidant and thrombolytic. The compounds elucidated by spectroscopic and mass methods were: (1) α-amyrin; (2) β-amyrin, (3) triterpene mix of α and β-amyrin acetate, (4) β-sitosterol, (5) Caryophyllene oxide, (6) γ-Muurolene, (7) Calamenene, (8) Caryophyllene, (9) α-Selinene, (10) Globulol, (11) Spathulenol, (12) δ-Cadinol, (13) β-Eudesmol and (14) α-Eudesmol. The ethanolic extract of Eugenia pruniformis (EEPP) displayed activity in both antioxidant tests - DPPH and ABTS with EC50= 12.60 ± 1.08 and 1.30 ± 2.05 μg/mL, respectively. Trolox used as a standard, presented EC50= 3.90± 1.20 and 0.97 ± 1.40 μg/mL, respectively. EEEP promoted 14.40 ± 7.00% of clot lysis. The results proved the presence of bioactive in the Eugenia pruniformis leave’s extract with antioxidant and thrombolytic activities. The present work is the first to report certain chemical and biologic aspects of this species.

Keywords: Myrtaceae, Eugenia pruniformis; Phytochemical, Terpenes; Biological activity.