Abstract

GENOTOXIC EFFECTS OF DEPAKINE® AND/OR EPANUTIN® ON MALE ALBINO MICE MUS MUSCULUS: CYTOLOGICAL AND MOLECULAR STUDY

Nagla Zaky Ibrahim El -Alfy, Mahmoud Fathy Mahmoud, Amany Ibrahim Alqosaibi and Ayman Mohammad Abdullah*

ABSTRACT

Current study was planned to investigate the genotoxicity of the widely prescribed drugs depakine and/or epanutin in mice. Chromosomal aberrations were investigated by colchicine-hypotonic technique. In addition, semi-quantitative RT-PCR was applied to estimate the level of hepatic NQ01 and BCL-2 gene expression. This experiment was applied on four sections of male albino mice as control section, depakine treated section, epanutin treated section and section treated with depakine together with epanutin. It was found that all treated groups exhibited high significant increase in mean of total aberrations and variable degrees of regressions in hepatic NQ01 and BCL-2 gene expression values. Centromeric attenuation, chromatid gaps and deletions were the common structural aberrations in all treated groups. Moreover, polyploidy was only type of numerical aberration observed in all treated groups. it was concluded that human therapeutic dose range of depakine and/or epanutin induced high significant level of genotoxicity in mice without dose or time dependent.

Keywords: epilepsy, chromosome, liver, phenytoin, sodium valproate.