Abstract

EFFECT OF FEW FORMULATION VARIABLES ON ENTRAPMENT EFFICIENCY OF SILVER SULFADIAZINE NIOSOMES

Sanket Dharashivkar*, Sangeeta Sahasrabuddhe and Ashok Saoji

ABSTRACT

Conventional formulation of silver sulfadiazine (SSD) available in market has been shown to decrease wound-related infections and morbidity in burn wounds but required to be applied two to four times a day. Niosomally encapsulated SSD can prove beneficial to obtain a sustained release. SSD is not soluble in most of the polar and non polar solvents. Therefore, the objective of this study is to investigate the effect of organic and aqueous solvents used in the manufacturing of niosomes and some other formulation variables on the entrapment efficiency of SSD. Niosomes were developed by ethanol injection methods using span 60 and tween 60 with cholesterol. Formed niosomes were evaluated for entrapment efficiency, vesicle size, photomicroscopy, in vitro release, scanning electron microscopy, in vitro antimicrobial activity and stability. Different formulation factors were found to affect the entrapment efficiency. Optimized niosomes had shown the sustained release of 98.14 % over 28 hours. In vitro antimicrobial study using Staphylococcus aureus revealed that the niosomal formulation of SSD shows a better zone of inhibition (16 mm) in comparison to conventional dosage form (14 mm) even when used in half the concentration of conventional dosage form.

Keywords: Silver sulfadiazine, burn treatment, antimicrobial study, entrapment efficiency.