Abstract

STUDY OF THE IN VIVO AND IN VITRO TOXICITY OF AQUEOUS EXTRACT OF AGERATUM CONYZOIDES LINN. IN WISTAR RAT AND HUMAN CELLS

Okémy Andissa N.*, Kouloungous Bafila C.B.D., Nianga C., Mouankie J.B., Moussoungou A.S.U., Moulari B. and Abena A. A.

ABSTRACT

This research study on the aqueous extract of the fresh leaves of Ageratum conyzoides Linn. was conducted to assess its toxic potential in wistar rats and human cells. The experiments were carried out by conventional tests according to OECD criteria. The aqueous extract of Ageratum conyzoïdes Linn is administered intragastric in wistar rats at 2000-5000 mg/kg doses, the sub-chronic toxicity test and cytotoxicity by the MTT test (3[4,5-Dimethythiazol-2Yl] -diphenyltetrazolium bromide) was used. The tesults obtained showed that the aqueous extract of Ageratum conyzoides Linn up to the dose of 5000mg/kg is not lethal. In addition, this extract no longer changes the levels of white blood cells, red blood cells, hemoglobin, platelets and no more creatinine, triglycerides, cholesterols, transaminases and alkaline phosphatases. On the other hand, from a cellular point of view, the aqueous extract of Ageratum conyzoïdes Linn. Toxic to hepatocytes at doses of 22 and 50 mg/ml and toxic from 50 and 100 mg/kg to human keratinocytes.

Keywords: Subchronic toxicity, cytotoxicity, hepatocyte, keratinocyte, A. conyzoides.