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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DACLATASVIR BY RP-HPLC METHOD

Prof. Pradip Patil* and Dr. M. Vamsi Krishna

ABSTRACT

A simple, rapid and accurate stability indicating RP-HPLC method was developed for the determination of Daclatsvir. The method was carried out using Column- Kromasil C18 (250×4.6mm, 5μ using ammonium acetate buffer and ACN (40:60, v/v), pH 4.7 as the mobile phase with detection at 335 nm and a flow rate of 1.0 ml/min. The retention time was found to be 6.69 min. The responses were linear in concentrations range of 1-160 μg/ml with correlation coefficient of 0.999. The method was statistically validated for accuracy, precision, linearity, and robustness.

Keywords: Daclatasvir, RP-HPLC Methods, Validation.

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