Abstract

QUANTITATIVE DETERMINATION OF MANGIFERIN IN METHANOL EXTRACT OF BACANG MANGO (MANGIFERA FOETIDA L.) LEAVES BY THIN-LAYER CHROMATOGRAPHY DENSITOMETRY

Roslinda Rasyid, Rustini Ruslan, Syifa Mawaddah and Harrizul Rivai*

ABSTRACT

Bacang Mango (Mangifera foetida L.) is one of the mango species from the Anacardiaceae family. Mangiferin is an isolated compound from the genus Mangifera. Mangiferin has pharmacological effects such as antimicrobial, anti-inflammatory, antioxidant, and anticancer. The purpose of this research is to determine the levels of mangiferin in methanol extract of bacang mango leaves. Levels of mangiferin in methanol extract of bacang mango leaves are analyzed using validated Thin-Layer Chromatography (TLC) Densitometry method. Mangiferin levels are determined using TLC silica gel 60 GF254 plates as stationary phase and using a mobile phase of ethyl acetate: distilled water: formic acid (8.5: 1.5: 1 v/v). This analysis finds out that an Rf is 0.66. The analysis is done using densitometry at 257 nm wavelength. The validation of the analytical method shows linearity with correlation coefficient value 0.9997. Detection limits and quantification limits are 9.90 μg/mL and 32.98 μg/mL, respectively. Precision is indicated by a coefficient with a range of value 0.12 - 0.91%. Accuracy is obtained by percent recovery with a range of value 96.45 - 99.95%. Analysis mangiferin levels in methanol extract of bacang mango leaves show a percentage of levels around 0.28 ± 0.004%.

Keywords: Mangifera foetida L., Mangiferin, Validation of Analytical Method, TLC-Densitometry.