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A RP-HPLC METHOD DEVLOPMENT AND VALIDATION OF GLIMEPIRIDE AND PIOGLITAZONE FROM THE FORMULATION
*Komal N. Dhaigude and Dyade G. K.
ABSTRACT A RP-HPLC method was developed and validated for the estimation of pioglitazone, glimepiride in sample tablet. RP-HPLC analysis was performed with prominence liquid chromatography SPD 20-A/SPD-20A HPLC system equipped with a UV/VIS Shimadzu 1700 detector. The procedure for the estimation of glimepiride and pioglitazone was carried out a phenomenex C18 (250mm ×4.6mm ID, 5μm) column and detection wavelength at 228nm and 267.5nm for glimepiride and pioglitazone simultaneously. Mobile phase containing methanol: water (95:5) %v/v. was pumped through column at flow rate of 0.8ml/min. T mp tu w m i t i t 40˚ . Optimiz w v th f glimepiride and pioglitazone was 228nm and 267.5nm respectively. Retention time of glimepiride and pioglitazone were found to be 4.527 min and 3.905 min. %RSD of the glimepiride and pioglitazone were and found to be 1.38 and 0.158. %Recovery of the glimepiride and pioglitazone were and found to be 100.09 and 100.04. Keywords: Method development, Validation, Glimepiride, pioglitazone. [Download Article] [Download Certifiate] |