CHARACTARIZATION OF CHEMICAL GROUPS AND STUDY OF ANTIOXIDANT, ANTIMICROBIAL AND THROMBOLYTIC ACTIVITIES OF APHANAMIXISPOLYSTACHYA (STEM BARK)
Mohammad Sakim Majumder, Dr. Mohammad SalimHossain, Mohammad NurulAmin, Md. *Mizanur Rahman Moghal, Shimul Banik, Mohammad Mobarak Hossain,
ABSTRACT
The present study was conducted to detect possible chemicals
(phytoconstituents), and investigate antioxidant, antimicrobial and
thrombolytic activities of the extract of Aphanamixispolystachyastem
bark.Phytochemical screening was carried out using the standard test
methods of different chemical group. For investigating the antioxidant
activity, two complementary test methods namely DPPH free radical
scavenging assay and total phenolic content determination were carried
out. For the evaluation of in vitro antimicrobial activity, disc diffusion
method, and to determine the thrombolytic activity, the method of
Prasad et al., 2007 with minor modifications were used. The bark
extracts were a rich source of phytochemicals. In DPPH free radical
scavenging test,the Carbon tetra chloride soluble fraction showed the highest free radical
scavenging activity with IC50 value 19.86μg/ml. while compared to that of the reference
standards ascorbic acid .Aphanamixispolystachyawas also found as a good source of total
phenolic contents.Morever, the extracts revealed broadspectrum antimicrobial activity at the
concentration of 400 μg/disc. By comparing with the negative control the mean clot lysis %
was significant (p value
Keywords: Phytochemical, antioxidant, antimicrobial, thrombolytic, Aphanamixispolystachya.
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