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Abstract

CHARACTARIZATION OF CHEMICAL GROUPS AND STUDY OF ANTIOXIDANT, ANTIMICROBIAL AND THROMBOLYTIC ACTIVITIES OF APHANAMIXISPOLYSTACHYA (STEM BARK)

Mohammad Sakim Majumder, Dr. Mohammad SalimHossain, Mohammad NurulAmin, Md. *Mizanur Rahman Moghal, Shimul Banik, Mohammad Mobarak Hossain,

ABSTRACT

The present study was conducted to detect possible chemicals (phytoconstituents), and investigate antioxidant, antimicrobial and thrombolytic activities of the extract of Aphanamixispolystachyastem bark.Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in vitro antimicrobial activity, disc diffusion method, and to determine the thrombolytic activity, the method of Prasad et al., 2007 with minor modifications were used. The bark extracts were a rich source of phytochemicals. In DPPH free radical scavenging test,the Carbon tetra chloride soluble fraction showed the highest free radical scavenging activity with IC50 value 19.86μg/ml. while compared to that of the reference standards ascorbic acid .Aphanamixispolystachyawas also found as a good source of total phenolic contents.Morever, the extracts revealed broadspectrum antimicrobial activity at the concentration of 400 μg/disc. By comparing with the negative control the mean clot lysis % was significant (p value

Keywords: Phytochemical, antioxidant, antimicrobial, thrombolytic, Aphanamixispolystachya.


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