PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDIES ON ACHYRANTHES ASPERA
Jitendra bajaj1, *Vivek Dave2, Swapnil Sharma2, Ajay Shukla3, R.D. chakole4
1Department of Pharmacy, NRI Institute of Pharmacy, Bhopal, M.P., India
2Department of Pharmacy, Banasthali University, Banasthali, Raj., India.
3Department of Pharmacy, G. I.T, Jabalpur, M.P., India
4Department of Pharmacy, Govt. Polytechnic College, Amravati, Maharashtra, India
ABSTRACT
The powder of aerial parts of Achyranthes aspera was screened for
pharmacognostic character, fluorescence analysis and histochemical
analysis. This powder was also processed for determination of
qualitative and quantitative of some phytochemical constituents.
Pharmacognostical studies comprised of taxonomical characters,
macro and microscopical characters of the part used histochemical
tests and diagnostic characters were performed. Physicochemical
constants such as ash, extractive values and moisture content were
determined. The fluorescence analysis was done by treating
moderately coarse powder with different reagents and observed under
visible and ultra violet light (short wavelength and long wavelength).
Quantitative and qualitative phytochemical analyses were also
performed to confirm active constituent in the extract. The
physiochemical analysis showed that the aerial part of this plant contained ash values 10 % in
which acid-insoluble ash is 1.49% and water soluble is 6.5% moisture content is 200 mg/100
gm. The extracts were subjected to qualitative screening test for various constituents. This
revealed the presence of alkaloids, tannins, saponins, flavonoids, glycosides, phenolic
compounds, terpenoids and steroids. Quantitative analysis is also performed in methanolic extracts in which total glycosides, total alkaloid, total saponin and total flavonoids were
found to be 9%, 2% , 0.4% 1% respectively.
Keywords: Achyranthes aspera, methanolic extract, qualitative screening, quantitative analysis.
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