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CYTOTOXICITY OF RAMIE (Boehmeria nivea L. Gaud.) LEAVES EXTRACT ON MCF-7 BREAST CANCER CELL LINE
Annisa Abdiwijaya Qaromah*, Asri Peni Wulandari, Desi Harneti
ABSTRACT
The objective of this study was to investigate the cytotoxicity effect of the crude extract and fractions of Ramie (Boehmeria nivea L. Gaud.) leaves extract on in vitro MCF-7 breast cancer cell line. In vitro cytotoxicity were evaluated by MTT-based assays. The percentage of cell inhibition was analyzed using probit analysis to obtain 50% inhibitory concentration (IC50). Morphological alteration of the cell line after exposure with extract were observed under inverted microscope. The cytotoxicity assay revealed that the ethanol extract and the methylene chloride fraction from the ramie leaves exhibited marked anticancer activity and has strong antiproliferative with IC50 of 3.27 μg/mL and 3.79 μg/mL, respectively, on MCF-7 cancer cell line. Morphological alteration of the cell line after exposure with Boehmeria nivea L. Gaud. extract were observed under inverted microscope in the Keywords: Cytotoxicity, Boehmeria nivea L. Gaud., fractions, MTT assay. [Download Article] [Download Certifiate] |