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SIMULTANEOUS DETERMINATION OF AMILORIDE HYDROCHLORIDE AND TORSEMIDE IN COMBINED PHARMACETICAL DOSAGE FORM USING SPECTROPHOTOMETRIC METHODS
Patel Grishma B.*, Hemant Patel
ABSTRACT Two simple, rapid and precise UV spectroscopic methods namely Simultaneous equation (method 1), Q-absorbance ratio (method 2) and have been developed for the simultaneous determination of Amiloride HCl (AML) and Torsemide (TOR). In method 1, both the drugs exhibit good linearity over the concentration range of 2-14 μg/ml and 4-28 μg/ml of AML and TOR at 361(r2=0.9996 and 0.9993 respectively) and 285 (r2=0.998 and 0.9993 respectively) wavelengths respectively. Method 2 involves the formation of Q-absorbance equation using the absorptivity values at 330.0 nm (isoabsorptive point) (r2=0.9993 and 0.999 respectively) and 285 nm (λmax of TOR) (r2=0.9992 and 0.9991 respectively) and Beer’s Lambert’s law was obeyed over the concentration range of 2-14 μg/ml and 4-28 μg/ml respectively. The proposed methods were validated according to ICH guidelines for evaluation of accuracy, precision, sensitivity etc. In conclusion, the proposed methods are novel, simple, accurate, precise, sensitive, rapid and economically viable methods that do not require any prior separation procedure. The proposed two methods hold potential for simultaneous determination of AML and TOR in tablet dosage form. Keywords: Amiloride HCl (AML), Torsemide (TOR), Spectrophotometric method, simultaneous Equation method, Absorption ratio method, validation. [Download Article] [Download Certifiate] |